recombinant human fgf-basic peprotech 100-18b  (PeproTech)

Journal: Cell Reports Medicine

Article Title: Screening of patient-derived organoids identifies mitophagy as a cell-intrinsic vulnerability in colorectal cancer during statin treatment

doi: 10.1016/j.xcrm.2025.102039

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Article Snippet: Organoids were cultured according to established protocols., , , , The CRC organoids culture medium consisted of Advanced Dulbecco’s Modified Eagle Medium/F12 (Gibco, 12634028) supplemented with 10 mM HEPES (Gibco, 15630080), 2 mM GlutaMAX (Gibco, 35050061), 1× Primocin (InvivoGen, ant-pm-05), 1× B-27 Supplement (Gibco, 17504044), 1 mM N-acetyl-L-cysteine (Sigma-Aldrich, A9165), 50 ng/mL EGF (Gibco, PMG8041), 100 ng/mL Noggin (PeproTech, 250-38), 10% R-spondin 1 conditioned medium, 10 nM gastrin I (Tocris, 3006), 10 mM Nicotinamide (Sigma-Aldrich, N0636), 500 nM A 83-01 (Tocris, 2939), 100 ng/mL IGF-I (BioLegend, 590908), 50 ng/mL FGF-basic (PeproTech, 100-18B), and 10 nM Prostaglandin E2 (Sigma-Aldrich, P6532).

Techniques: Formalin-fixed Paraffin-Embedded, Recombinant, Modification, Cell Recovery, Concentration Assay, Cell Culture, Viability Assay, Clinical Proteomics, Membrane, Software

Journal: Cell Reports Medicine

Article Title: Self-assembled patient-derived tumor-like cell clusters for personalized drug testing in diverse sarcomas

doi: 10.1016/j.xcrm.2025.101990

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Article Snippet: FGF-basic , Peprotech , 100-AF-18B.

Techniques: Recombinant, Staining, Imaging, RNA Expression, Software, Variant Assay